Flaxseed Oil as a Novel Anti-Epidermal Growth Factor Receptor Agent: Enhancing Cisplatin Efficacy in Squamous Cell Carcinoma Treatment
DOI:
https://doi.org/10.5281/zenodo.15094146Keywords:
Flaxseed oil, Squamous cell carcinoma, Cisplatin, Epidermal Growth Factor Receptors, Natural Therapeutics.Abstract
Squamous cell carcinoma represents a widespread form of cancer that demands new innovative and effective therapeutic strategies to address its growth concern. This research investigates whether flaxseed oil functions as a supplemental therapeutic agent which improves the effectiveness of cisplatin against squamous cell carcinoma (SCC) cell lines particularly using the OECM-1 cell line. The experimental design involved quantifying SCC25 cell growth rates by investigating epidermal growth factor receptor activity in 96-well plates as the primary means to study biological process activity. The cell treatment consisted of studying flaxseed oil and cisplatin alone and combined to determine their cytotoxic effects. The IC50 values represent the concentration of the compound required to inhibit 50% of the epidermal growth factor receptors (EGFR) activity in SCC25 cells. The statistical tests involving one-way ANOVA and paired sample t-tests allowed measurement of cell viability at 24 and 48 hours after treatment. Results indicate that cells treated with flaxseed oil experienced a major decrease in cell viability from 46.28 to 29.14 (p = 0.007) and received similar reductions as cells treated with cisplatin which declined from 23.10 to 16.22 (p = 0.032). A remarkable change from 35.06 to 20.76 (p = 0.008) was observed through combined administration. Mean values within the control group dramatically increased to reflect the success of the treatments applied. This research establishes that therapeutic potential of flaxseed oil when paired with cisplatin treatment for squamous cell carcinoma. The research indicates that implementing natural compounds alongside conventional therapies leads to superior therapeutic outcomes and diminished side effects. Research initiatives should concentrate on detailed investigations of synergistic processes alongside clinical performance assessments for combined cancer treatments in medical settings.
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